Name: IWP-2
Brand: Selleck Chemicals
SKU: S7085
Rating: 5 (136 reviews)

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Control de calidad

Lote: Pureza: 99.60%

99.60

Productos que se suelen utilizar junto con IWP-2

IWP-4

It and IWP-4 are WNT inhibitors that are commonly used as small molecules for human cardiomyocytes (CMs) differentiation.

LGK974 (WNT974)

This compound and LGK-974 decrease both sphere formation and transcriptional activities of CD44 in both RWPE-1 and PZ-HPV7 knock down (KD) cells.

PRI-724 (Foscenvivint)

It and PRI-724 induce apoptosis, affect HNSCC cell migration, and strongly affect β-catenin-dependent gene expression in HNSCC cells.

Dianas relacionadas	JAK TGF-beta/Smad ERK GSK-3 ROCK Hedgehog/Smoothened PKA Secretase STAT Casein Kinase
Otros Wnt/beta-catenin Inhibidores	IWR-1-endo Tegatrabetan (BC-2059) Isoquercitrin ICG-001 SKL2001 BML-284 Hydrochloride (Wnt agonist 1, AMBMP) PNU-74654 Salinomycin (Procoxacin) FH535 LF3

Cultivo celular, tratamiento y concentración de trabajo

Líneas celulares	Tipo de ensayo	Tiempo de incubación	Descripción de la actividad	PMID
mouse L cells	Function assay	24 h	Inhibition of porcupine-mediated Wnt signalling in mouse L cells after 24 hrs by SpringerImages-Topflash reporter assay, EC50=30 nM	23477365
HEK293T	Function assay	22 hrs	Inhibition of porcupine in HEK293T cells transfected with Wnt3A-expressing vector assessed as suppression of Wnt/beta-catenin signaling after 22 hrs by Super-top flash reporter gene assay, IC50 = 0.157 μM.	29630366
MIAPaCa2	Antiproliferative assay	48 hrs	Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by MTT assay, EC50 = 1.9 μM.	29630366
SW620	Antiproliferative assay	48 hrs	Antiproliferative activity against human SW620 cells after 48 hrs by MTT assay, EC50 = 1.9 μM.	29630366
Capan1	Antiproliferative assay	48 hrs	Antiproliferative activity against human Capan1 cells after 48 hrs by MTT assay, EC50 = 2.05 μM.	29630366
PANC1	Antiproliferative assay	48 hrs	Antiproliferative activity against human PANC1 cells after 48 hrs by MTT assay, EC50 = 2.33 μM.	29630366
HEK293	Antiproliferative assay	48 hrs	Antiproliferative activity against human HEK293 cells after 48 hrs by MTT assay, EC50 = 2.76 μM.	29630366
Panc89	Antiproliferative assay	48 hrs	Antiproliferative activity against human Panc89 cells after 48 hrs by MTT assay, EC50 = 3.86 μM.	29630366
HT-29	Antiproliferative assay	48 hrs	Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay, EC50 = 4.67 μM.	29630366
A818-6	Antiproliferative assay	48 hrs	Antiproliferative activity against human A818-6 cells after 48 hrs by MTT assay, EC50 = 8.96 μM.	29630366
A673	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
DAOY	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
Saos-2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-12	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
OHS-50	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
BT-12	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells	29435139
DAOY	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for DAOY cells	29435139
Rh41	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells	29435139
A673	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells)	29435139
MG 63 (6-TG R)	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells	29435139
fibroblast cells	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for control Hh wild type fibroblast cells	29435139
Rh41	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Rh41 cells	29435139
SJ-GBM2	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
LAN-5	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells	29435139
TC32	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells	29435139
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Información química, almacenamiento y estabilidad

Peso molecular	466.6	Fórmula	C₂₂H₁₈N₄O₂S₃	Almacenamiento (Desde la fecha de recepción)	3 años-20°Cpolvo
Nº CAS	686770-61-6	Descargar SDF		Almacenamiento de soluciones madre	1 año-80°Cen disolvente 1 mes-20°Cen disolvente
Sinónimos	N/A			Smiles	CC1=CC2=C(C=C1)N=C(S2)NC(=O)CSC3=NC4=C(C(=O)N3C5=CC=CC=C5)SCC4

Solubilidad

In vitro
Lote:

DMSO : 20 mg/mL (42.86 mM) Calentado con baño de agua a 50°C; Ultrasonido;
(El DMSO contaminado con humedad puede reducir la solubilidad. Usar DMSO fresco y anhidro.)

DMF : 12.5 mg/mL

Water : Insoluble

DMF : 20 mg/mL

DMSO : 3 mg/mL (6.42 mM) Calentado con baño de agua a 50°C; Ultrasonido;
(El DMSO contaminado con humedad puede reducir la solubilidad. Usar DMSO fresco y anhidro.)

Water : Insoluble

Calculadora de Molaridad

Masa	Concentración	Volumen	Peso molecular
=	×	×

Calculadora de Dilución Calculadora de Peso Molecular

In vivo Lote:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Calculadora de formulación in vivo (Solución clara)

Paso 1: Introduzca la información a continuación (Recomendado: Un animal adicional para tener en cuenta la pérdida durante el experimento)

Dosis: mg/kg Peso promedio de los animales: g Volumen de dosificación por animal:μL Número de animales:

Paso 2: Introduzca la formulación in vivo (Esto es solo la calculadora, no la formulación. Por favor, contáctenos primero si no hay una formulación in vivo en la sección de Solubilidad.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Resultados del cálculo:

Concentración de trabajo: mg/ml;

Método para preparar el líquido maestro de DMSO: mg fármaco predissuelto en μL DMSO ( Concentración del líquido maestro mg/mL, Por favor, contáctenos primero si la concentración excede la solubilidad del DMSO del lote del fármaco. )

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadirμL PEG300, mezclar y clarificar, luego añadirμL Tween 80, mezclar y clarificar, luego añadir μL ddH₂O, mezclar y clarificar.

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadir μL Aceite de maíz, mezclar y clarificar.

Nota: 1. Por favor, asegúrese de que el líquido esté claro antes de añadir el siguiente disolvente.
2. Asegúrese de añadir el (los) disolvente(s) en orden. Debe asegurarse de que la solución obtenida, en la adición anterior, sea una solución clara antes de proceder a añadir el siguiente disolvente. Se pueden utilizar métodos físicos como el vórtice, el ultrasonido o el baño de agua caliente para ayudar a la disolución.

Mecanismo de acción

Características	Selective inhibitor of Porcn-mediated Wnt secretion.
Targets/IC₅₀/Ki	Wnt (Cell-free assay) 27 nM ^M82FCK1δ (Cell-free assay) 40 nM
In vitro	IWP-2 es útil tanto en medicina regenerativa como en los esfuerzos contra el cáncer. Este compuesto inactiva Porcn, una O-aciltransferasa unida a la membrana (MBOAT), e inhibe selectivamente la palmitoilación de Wnt. Bloquea la fosforilación dependiente de Wnt del receptor Lrp6 y Dvl2, y la acumulación de β-catenina.
Referencias	[1] https://pubmed.ncbi.nlm.nih.gov/19125156/ [2] https://pubmed.ncbi.nlm.nih.gov/25536657/ [3] https://pubmed.ncbi.nlm.nih.gov/29630366/

Aplicaciones

Métodos	Biomarcadores	Imágenes	PMID
Western blot	Wnt5-a/b / β-catenin / Axin2 c-myc / Cyclin D1 / Survivin MMP7		27119499

Soporte técnico

Instrucciones de manipulación

Tel: +1-832-582-8158 Ext:3

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IWP-2 inhibidor de la vía WNT

Estructura química

Peso molecular: 466.6