Sodium ascorbate Vitamin chemical

Sodium ascorbate has a growth inhibiting action only at high concentrations in cultured human neoplastic cell lines MCF-7 (breast carcinoma), KB (oral epidermoid carcinoma), and AN3-CA (endometrial adenocarcinoma). This compound combined with vitamin K3 demonstrates a synergistic inhibition of cell growth at 10 to 50 times lower concentrations in cultured human neoplastic cell lines MCF-7, KB, and AN3-CA, at this level separately given vitamins are not toxic. This tumor cell growth inhibitory effect is completely suppressed by the addition of catalase to the culture medium containing vitamins C and K3, suggesting an excessive production of hydrogen peroxide as being implied in mechanisms responsible for the above-mentioned effects. [1] This chemical combined with vitamin K3 results in a synergistic effect on growth inhibition in cultured human endometrial adenocarcinoma (AN3CA) cells. [2] It results in a rapid increase in the intracellular concentration of Ca²⁺ ions and subsequent apoptotic cell death in HL-60 cells, characterized by cell shrinkage, nuclear fragmentation and cleavage of internucleosomal DNA to yield fragments that are multiples of 180-200 base pairs, are induced. [3] This compound (100 μM) induces DNA single-strand breaks in human cells, Fibroblasts and Molt-4 cells are significantly more sensitive than lymphocytes. It (50 μM) results in significant cell loss in Molt-4 cells, but not in lymphocyte and fibroblast cultures. [4]