Name: Hydrocortisone (Cortisol)
Brand: Selleck Chemicals
SKU: S1696
Rating: 5 (36 reviews)

Saltar a

Control de calidad

Lote: Pureza: 99.99%

99.99

Dianas relacionadas	Adrenergic Receptor Estrogen/progestogen Receptor GPR Androgen Receptor ACE RAAS Progesterone Receptor Opioid Receptor PGES THR
Otros Glucocorticoid Receptor Inhibidores	Corticosterone AL082D06 (20S)-Protopanaxatriol Cortodoxone

Cultivo celular, tratamiento y concentración de trabajo

Líneas celulares	Tipo de ensayo	Concentración	Tiempo de incubación	Descripción de la actividad	PMID
mouse RAW264.7 cells	Function assay		24 h	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction, IC50=0.061 μM	21361338
mouse L929 cells	Growth inhibition assay		6 days	Growth inhibition of mouse L929 cells after 6 days	926113
RAW264.7	Antiinflammatory assay			Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay, IC50 = 40.64 μM.	20879743
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry, IC50 = 40.64 μM.	21807513
RAW264.7	Function assay		24 hrs	Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction, IC50 = 40.64 μM.	23067550
RAW264.7	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50 = 43.8 μM.	23755850
L929	Function assay		20 hrs	Displacement of 1 x 10'-8 M of [1,2,3-3H]-triamcinolone acetonide from glucocorticoid receptor in soluble fraction of mouse L929 cells after 20 hrs, Kd = 0.043 μM.	926113
HEK293	Function assay			Inhibition of MR-mediated transactivation of galactosidase reporter gene in HEK293 cells expressing 11betaHSD1	16759088
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-10 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL-6 production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of IL1-beta production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of TNFalpha production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by ELISA relative to control	21384845
RAW264.7	Antiinflammatory assay	0.5 to 50 ug/ml	4 hrs	Antiinflammatory activity against LPS-stimulated mouse RAW264.7 cells assessed as inhibition of nitric oxide production at 0.5 to 50 ug/ml pretreated for 4 hrs followed by challenge with 1 ug/mL LPS for 48 hrs by Griess reaction method relative to control	21384845
neural precursor cells	Function assay			Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay	17417631
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in TNF-alpha level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-6 level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced increase in IL-1beta level at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs by ELISA relative to control	25515561
RAW264.7	Antiinflammatory assay	10 uM	2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production at 10 uM incubated for 2 hrs prior to LPS challenge measured after 22 hrs relative to vehicle-treated control	25515561
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in cleaved caspase 3 level at 20 uM after 36 hrs by western blot analysis	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in p53 expression at 20 uM after 36 hrs by propidium iodide staining based FACS analysis	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increased Bax expression at 20 uM after 36 hrs by western blot analysis	26695732
B16F10	Antitumor activity assay	6.11 mg/kg		Antitumor activity against mouse B16F10 cells xenografted in C57BL/J mouse assessed as inhibition of tumor growth at 6.11 mg/kg, ip	26695732
B16F10	Apoptosis assay	20 uM	36 hrs	Induction of apoptosis in mouse B16F10 cells assessed as increase in cytochrome C level at 20 uM after 36 hrs by western blot analysis	26695732
Haga clic para ver más datos experimentales de líneas celulares

Información química, almacenamiento y estabilidad

Peso molecular	362.46	Fórmula	C₂₁H₃₀O₅	Almacenamiento (Desde la fecha de recepción)	3 años-20°Cpolvo
Nº CAS	50-23-7	Descargar SDF		Almacenamiento de soluciones madre	1 año-80°Cen disolvente 1 mes-20°Cen disolvente
Sinónimos	NSC 10483			Smiles	CC12CCC(=O)C=C1CCC3C2C(CC4(C3CCC4(C(=O)CO)O)C)O

Solubilidad

In vitro
Lote:

DMSO : 72 mg/mL (198.64 mM)
(El DMSO contaminado con humedad puede reducir la solubilidad. Usar DMSO fresco y anhidro.)

Ethanol : 15 mg/mL

Water : Insoluble

Calculadora de Molaridad

Masa	Concentración	Volumen	Peso molecular
=	×	×

Calculadora de Dilución Calculadora de Peso Molecular

In vivo Lote:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Calculadora de formulación in vivo (Solución clara)

Paso 1: Introduzca la información a continuación (Recomendado: Un animal adicional para tener en cuenta la pérdida durante el experimento)

Dosis: mg/kg Peso promedio de los animales: g Volumen de dosificación por animal:μL Número de animales:

Paso 2: Introduzca la formulación in vivo (Esto es solo la calculadora, no la formulación. Por favor, contáctenos primero si no hay una formulación in vivo en la sección de Solubilidad.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Resultados del cálculo:

Concentración de trabajo: mg/ml;

Método para preparar el líquido maestro de DMSO: mg fármaco predissuelto en μL DMSO ( Concentración del líquido maestro mg/mL, Por favor, contáctenos primero si la concentración excede la solubilidad del DMSO del lote del fármaco. )

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadirμL PEG300, mezclar y clarificar, luego añadirμL Tween 80, mezclar y clarificar, luego añadir μL ddH₂O, mezclar y clarificar.

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadir μL Aceite de maíz, mezclar y clarificar.

Nota: 1. Por favor, asegúrese de que el líquido esté claro antes de añadir el siguiente disolvente.
2. Asegúrese de añadir el (los) disolvente(s) en orden. Debe asegurarse de que la solución obtenida, en la adición anterior, sea una solución clara antes de proceder a añadir el siguiente disolvente. Se pueden utilizar métodos físicos como el vórtice, el ultrasonido o el baño de agua caliente para ayudar a la disolución.

Mecanismo de acción

Targets/IC₅₀/Ki	Glucocorticoid receptor
In vitro	Hydrocortisone previene la ruptura de la barrera endotelial en respuesta a estímulos proinflamatorios (administración de TNFalfa) en la línea celular endotelial microvascular cerebral humana hCMEC/D3, lo que podría demostrarse que se basa en parte en el mantenimiento de los niveles de ocludina. Las células dendríticas (CD) tratadas con este compuesto muestran una expresión disminuida de las moléculas de MHC II, la molécula coestimuladora CD86 y el marcador específico de CD CD83, así como una secreción de IL-12 fuertemente reducida. Inhibe la producción de IFN-gamma pero induce una mayor liberación de IL-4 y ningún cambio en IL-5. Este químico reduce la proliferación de células T en las células dendríticas. Previene la degradación severa del glicocálix inducida por TNF-alfa, el aumento de la resistencia coronaria, el aumento de la fuga vascular y la permeabilidad al hidroxietilalmidón y causó la degranulación de mastocitos en corazones aislados de cobaya. Este compuesto reduce el estrés oxidativo postisquémico, la presión de perfusión y la formación de transudado en corazones aislados de cobaya. Inhibe el desprendimiento postisquémico de sindecán-1, sulfato de heparano y hialuronano, así como la liberación de histamina de los mastocitos residentes. Aumenta los niveles de transcritos de línea germinal C epsilon inducidos por IL-4 en dos veces y entrega la señal requerida para la transcripción de ARNm C epsilon maduro. Este químico induce la recombinación de cambio delecional S mu-S epsilon en células B tratadas con IL-4, y apoya un modelo de cambio secuencial de isotipo de IgM a IgE a través de IgG4.
Referencias	[1] https://pubmed.ncbi.nlm.nih.gov/18258663/ [2] https://pubmed.ncbi.nlm.nih.gov/11496241/ [3] https://pubmed.ncbi.nlm.nih.gov/18836678/ [4] https://pubmed.ncbi.nlm.nih.gov/18073553/ [5] https://pubmed.ncbi.nlm.nih.gov/8409416/

Aplicaciones

Métodos	Biomarcadores	PMID
Western blot	parkin / AIMP2 CREB	28366931
Immunofluorescence	Glut2	29717618
Growth inhibition assay	Cell viability	22829975

Información del ensayo clínico

(datos de https://clinicaltrials.gov, actualizado el 2024-05-22)

Número NCT	Reclutamiento	Condiciones	Patrocinador/Colaboradores	Fecha de inicio	Fases
NCT05741762	Active not recruiting	Critical Illness\|Adrenal Insufficiency\|Septic Shock	Dr Adnan Agha\|United Arab Emirates University	January 31 2023	--
NCT05607901	Recruiting	Dermatologic Disease	Tanta University	October 28 2022	Phase 2
NCT05324618	Completed	Atopic\|Dermatitis	Ain Shams University\|National Hepatology & Tropical Medicine Research Institute	May 15 2022	Phase 4

Soporte técnico

Instrucciones de manipulación

Tel: +1-832-582-8158 Ext:3

Si tiene alguna otra consulta, por favor deje un mensaje.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Hydrocortisone (Cortisol) Glucocorticoid Receptor agonista

Estructura química

Peso molecular: 362.46