CD163L1 Antibody [C10D1] | Anticuerpos primarios

Aplicación: Reactividad:Human

Información de uso

Dilución
IHC1:2000

Aplicación
IHC

Reactividad
Human

Fuente
Rabbit Monoclonal Antibody

Tampón de almacenamiento
PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Almacenamiento (desde la fecha de recepción)
-20°C (avoid freeze-thaw cycles), 2 years

Control positivo	Tonsil macrophages tissue; Human spleen tissue; Human stomach tissue; Lung macrophages tissue; Human uterus tissue; Stomach macrophages tissue; Liver macrophages tissue
Control negativo	Placenta tissue

Métodos experimentales

IHC
Experimental Protocol： Deparaffinization/Rehydration 1. Deparaffinize/hydrate sections: 2. Incubate sections in three washes of xylene for 5 min each. 3. Incubate sections in two washes of 100% ethanol for 10 min each. 4. Incubate sections in two washes of 95% ethanol for 10 min each. 5. Wash sections two times in dH2O for 5 min each. 6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min. Staining 1. Wash sections in dH2O three times for 5 min each. 2. Incubate sections in 3% hydrogen peroxide for 10 min. 3. Wash sections in dH2O two times for 5 min each. 4. Wash sections in wash buffer for 5 min. 5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature. 6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C. 7. Remove antibody solution and wash sections with wash buffer three times for 5 min each. 8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature. 9. Wash sections three times with wash buffer for 5 min each. 10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use. 11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity. 12. Immerse slides in dH2O. 13. If desired, counterstain sections with hematoxylin. 14. Wash sections in dH2O two times for 5 min each. 15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each. 16. Mount sections with coverslips and mounting medium.

IHC

Experimental Protocol：

Deparaffinization/Rehydration

1. Deparaffinize/hydrate sections:

2. Incubate sections in three washes of xylene for 5 min each.

3. Incubate sections in two washes of 100% ethanol for 10 min each.

4. Incubate sections in two washes of 95% ethanol for 10 min each.

5. Wash sections two times in dH2O for 5 min each.

6.Antigen retrieval: For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; continue with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.

Staining

1. Wash sections in dH2O three times for 5 min each.

2. Incubate sections in 3% hydrogen peroxide for 10 min.

3. Wash sections in dH2O two times for 5 min each.

4. Wash sections in wash buffer for 5 min.

5. Block each section with 100–400 µl of blocking solution for 1 hr at room temperature.

6. Remove blocking solution and add 100–400 µl primary antibody diluent in to each section. Incubate overnight at 4°C.

7. Remove antibody solution and wash sections with wash buffer three times for 5 min each.

8. Cover section with 1–3 drops HRPas needed. Incubate in a humidified chamber for 30 min at room temperature.

9. Wash sections three times with wash buffer for 5 min each.

10. Add DAB Chromogen Concentrate to DAB Diluent and mix well before use.

11. Apply 100–400 µl DAB to each section and monitor closely. 1–10 min generally provides an acceptable staining intensity.

12. Immerse slides in dH2O.

13. If desired, counterstain sections with hematoxylin.

14. Wash sections in dH2O two times for 5 min each.

15. Dehydrate sections: Incubate sections in 95% ethanol two times for 10 sec each; Repeat in 100% ethanol, incubating sections two times for 10 sec each; Repeat in xylene, incubating sections two times for 10 sec each.

16. Mount sections with coverslips and mounting medium.

Datasheet & SDS

Descripción biológica

Especificidad
CD163L1 Antibody [C10D1] detects endogenous levels of total CD163L1 protein.

Localización subcelular
Cell membrane, Membrane, Secreted

Uniprot ID
Q9NR16

Clon
C10D1

Sinónimo
CD163b; CD163B; M160; UNQ6434/PRO23202; CD163L1; Scavenger receptor cysteine-rich type 1 protein M160; CD163 antigen-like 1

Antecedentes
CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

Antecedentes

CD163L1, also known as CD163 molecule-like 1 or M160, is a type I membrane protein of the group B scavenger receptor cysteine-rich (SRCR) superfamily that features multiple SRCR domains (typically 9–12), a single transmembrane segment, and a cytoplasmic tail that mediates endocytosis via a clathrin-dependent pathway independent of ligand cross-linking. Arising from a late evolutionary duplication of the CD163 gene, CD163L1 is highly expressed on specific tissue macrophage subsets (often colocalizing with CD163), but is minimally present or absent in monocytes, alveolar macrophages, glia, and Kupffer cells; two cytoplasmic splice variants further influence its subcellular localization. CD163L1 serves as an endocytic scavenger receptor likely binding as-yet unidentified ligands to mediate clearance and promote resolution of inflammation through anti-inflammatory signaling and maintenance of tissue homeostasis, differentiating it from CD163, which specifically scavenges hemoglobin–haptoglobin complexes. CD163L1 shows no affinity for hemoglobin–haptoglobin or tested bacteria, and does not form known protein complexes. Its regulated expression during monocyte-to-macrophage differentiation supports innate immune modulation, with disease relevance in inflammatory conditions such as atherosclerosis, potentially through oxidant clearance akin to CD163, and in autoimmune contexts by dampening excessive immune responses.

Referencias
https://pubmed.ncbi.nlm.nih.gov/22900885/ https://pubmed.ncbi.nlm.nih.gov/22279103/

Tabla de referencia para seleccionar las especificaciones del tamaño de poro de la membrana PVDF

Tamaño de la proteína	Tamaño de poro de la membrana de transferencia PVDF
< 10	0.22
10-20	0.22
20-30	0.45
30-45	0.45
45-70	0.45
80-100	0.45
100-140	0.45
> 140	0.45

Soporte técnico

Instrucciones de manipulación

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Tamaño de la proteína	Porcentaje de gel de separación
4-40	20%
12-45	15%
10-70	12.5%
15-100	10%
25-100	8%
60-210	5%