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Nocodazole Microtubule Depolymerization inhibitor

Name: Nocodazole
Brand: Selleck Chemicals
SKU: S2775
Rating: 5 (130 reviews)

N° Cat.S2775

Nocodazole est un inhibiteur rapidement réversible de la polymérisation des microtubules, inhibe également Abl, Abl(E255K) et Abl(T315I) avec un IC50 de 0,21 μM, 0,53 μM et 0,64 μM respectivement dans des essais acellulaires. Nocodazole induit l'apoptosis.

Nocodazole Microtubule Associated inhibiteur Chemical Structure

Structure chimique

Poids moléculaire: 301.32

Aller à

Contrôle qualité

Lot : Pureté : 99.80%

99.80

Cibles apparentées	Akt Wnt/beta-catenin PKC HSP ROCK Integrin Bcr-Abl Actin FAK Kinesin
Autre Microtubule Associated Inhibiteurs	MMAF Patupilone (Epothilone B) Lexibulin (CYT997) CW069 Combretastatin A4 Epothilone A ABT-751 (E7010) TAI-1 Cucurbitacin B INH1

Culture cellulaire, traitement et concentration de travail

Lignées cellulaires	Type dessai	Concentration	Temps dincubation	Description de lactivité	PMID
L1210 murine leukemia cell	Growth inhibition assay			In vitro for its inhibitory activity against L1210 murine leukemia cell growth, IC50=38 nM	1738146
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.006 μM.	19220018
HepG2	Function assay	0.1 to 10 uM	1 hr	Inhibition of TNFalpha-induced NFkappaB (unknown origin)-dependent transcriptional activity expressed in human HepG2 cells at 0.1 to 10 uM incubated for 1 hr prior to TNFalpha induction measured after 6 hrs by dual-luciferase reporter gene assay	25827522
PANC1	Antiproliferative assay		48 hrs	Antiproliferative activity against human PANC1 cells after 48 hrs by SRB assay, GI50 = 0.01 μM.	27688193
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.01 μM.	27688193
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.01 μM.	27688193
A549	Antiproliferative assay		48 hrs	Antiproliferative activity against human A549 cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM.	29089233
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM.	29089233
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM.	29089233
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.015 μM.	25827522
MDA-MB-231	Antiproliferative assay		48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.017 μM.	25827522
A549	Antiproliferative assay		48 hrs	Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.02 μM.	25827522
L1210	Function assay		48 hrs	Inhibitory concentration against proliferation of cultured lymphoid leukemia L1210 cells during 48 hrs, ID50 = 0.027 μM.	7131483
PANC1	Anticancer assay		48 hrs	Anticancer activity against human PANC1 cells after 48 hrs by SRB assay, GI50 = 0.029 μM.	27130357
A549	Cytotoxicity assay		24 hrs	Cytotoxicity against human A549 cells after 24 hrs by MTT assay, IC50 = 0.0366 μM.	20110137
NCI60	Growth inhibition assay		48 hrs	Growth inhibition of human NCI60 cells incubated for 48 hrs by sulforhodamine B assay, GI50 = 0.0389 μM.	27956038
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.04 μM.	19220018
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against LT12 cells by XTT assay after 48 hrs, IC50 = 0.04 μM.	17181164
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.04 μM.	20537765
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.04 μM.	21705223
MDA-MB-231	Anticancer assay		48 hrs	Anticancer activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.042 μM.	27130357
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against L1210 cells by XTT assay after 48 hrs, IC50 = 0.05 μM.	17181164
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse adriamycin-resistant P388 cells after 48 hrs by XTT assay, IC50 = 0.05 μM.	17973361
L12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat multidrug-resistant L12 cells after 48 hrs by XTT assay, IC50 = 0.05 μM.	17973361
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.05 μM.	20537765
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against ADR-resistant mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.05 μM.	21705223
U2OS	Function assay		72 hrs	Induction of mitotic arrest in human U2OS cells assessed as mitotic index after 72 hrs by microscopic analysis, EC50 = 0.05 μM.	24900887
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM.	17973361
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM.	19220018
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM.	21705223
HeLa	Anticancer assay		48 hrs	Anticancer activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.063 μM.	27130357
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.065 μM.	28117204
NCI-H460	Antiproliferative assay		48 hrs	Antiproliferative activity against human NCI-H460 cells after 48 hrs by alamar blue assay, IC50 = 0.068 μM.	21563750
BT549	Antiproliferative assay		48 hrs	Antiproliferative activity against human BT549 cells after 48 hrs by alamar blue assay, IC50 = 0.069 μM.	21563750
LT12 MDR	Antiproliferative assay		48 hrs	Antiproliferative activity against LT12 MDR cells by XTT assay after 48 hrs, IC50 = 0.07 μM.	17181164
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse vincristine-resistant L1210 cells after 48 hrs by XTT assay, IC50 = 0.07 μM.	17973361
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.07 μM.	17973361
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against multi drug-resistant rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.07 μM.	20537765
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse P388 cells after 48 hrs by XTT proliferation assay, IC50 = 0.07 μM.	21705223
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat multiple drug-resistant LT12 cells after 48 hrs by XTT assay, IC50 = 0.07 μM.	21705223
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse VCR-resistant L1210 cells after 48 hrs by XTT assay, IC50 = 0.07 μM.	21705223
451LU	Antiproliferative assay		48 hrs	Antiproliferative activity against human 451LU cells after 48 hrs by alamar blue assay, IC50 = 0.072 μM.	21563750
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against P388 cells by XTT assay after 48 hrs, IC50 = 0.08 μM.	17181164
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.08 μM.	20537765
SKOV3	Antiproliferative assay		48 hrs	Antiproliferative activity against human SKOV3 cells after 48 hrs by alamar blue assay, IC50 = 0.08 μM.	21563750
SGC7901	Growth inhibition assay		72 hrs	Growth inhibition of human SGC7901 cells after 72 hrs by MTT assay, IC50 = 0.08 μM.	25262051
A549	Anticancer assay		48 hrs	Anticancer activity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.08 μM.	27130357
K562	Function assay			Effective concentration required to arrest propidium iodide stained K562 cells in G2/M phase, EC50 = 0.082 μM.	12852768
SKBR3	Growth inhibition assay		48 hrs	Growth inhibition of human SKBR3 cells after 48 hrs by MTT assay, IC50 = 0.084 μM.	22850214
HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.086 μM.	28117204
A549	Cytotoxicity assay			Cytotoxicity against human A549 cells by MTT assay, IC50 = 0.087 μM.	21954851
COLO205	Antiproliferative assay		48 hrs	Antiproliferative activity against human COLO205 cells after 48 hrs by alamar blue assay, IC50 = 0.088 μM.	21563750
L1210 VCR	Antiproliferative assay		48 hrs	Antiproliferative activity against L1210 VCR cells by XTT assay after 48 hrs, IC50 = 0.09 μM.	17181164
L1210	Antiproliferative assay		48 hrs	Antiproliferative activity against vincristine-resistant mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.09 μM.	20537765
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.09 μM.	28117204
KB/HeLa	Cell cycle assay			Cell cycle arrest in KB/HeLa cells by accumulation at G2/M phase, EC50 = 0.091 μM.	17181164
KB/HeLa	Cell cycle assay		24 hrs	Cell cycle arrest in KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs by flow cytometric analysis, EC50 = 0.091 μM.	17973361
KB/HeLa	Cell cycle assay		24 hrs	Cell cycle arrest in human KB/HeLa cells assessed as G2/M phase accumulation after 24 hrs by FACS analysis, EC50 = 0.091 μM.	19220018
KB/HeLa	Cell cycle assay		24 hrs	Cell cycle arrest in human KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs using propidium iodide staining by FACS analysis, EC50 = 0.091 μM.	20537765
KB/HeLa	Cell cycle assay		24 hrs	Cell cycle arrest in human KB/HeLa cells assessed as accumulation of cells at G2/M phase after 24 hrs by flow cytometry, EC50 = 0.091 μM.	21705223
MiaPaCa	Cytotoxicity assay		48 hrs	Cytotoxicity against human MiaPaCa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.091 μM.	28117204
MCF7	Growth inhibition assay			Growth inhibition of human MCF7 cells by Sulforhodamine B assay, GI50 = 0.1 μM.	21106458
MCF7	Growth inhibition assay			Growth inhibition of human MCF7 cells by Sulforhodamine B assay, LC50 = 0.1 μM.	21106458
A549	Growth inhibition assay			Growth inhibition of human A549 cells by Sulforhodamine B assay, GI50 = 0.1 μM.	21106458
A549	Growth inhibition assay			Growth inhibition of human A549 cells by Sulforhodamine B assay, LC50 = 0.1 μM.	21106458
A549	Cell cycle assay		24 hrs	Cell cycle arrest in human A549 cells assessed as accumulation at G2/M phase after 24 hrs using propidium iodide staining by flow cytometry, EC50 = 0.1065 μM.	20110137
RKO	Cytotoxicity assay			Cytotoxic activity against human colon adenocarcinoma RKO cells without ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1, IC50 = 0.11 μM.	12852768
RKO	Antiproliferative assay		48 hrs	Antiproliferative activity against RKO cells by XTT assay after 48 hrs, IC50 = 0.11 μM.	17181164
RKO	Cytotoxicity assay		48 hrs	Cytotoxicity against human RKO cells after 48 hrs by XTT assay, IC50 = 0.11 μM.	17973361
RKO	Antiproliferative assay			Antiproliferative activity against human RKO cells by XTT assay, IC50 = 0.11 μM.	19220018
RKO	Antiproliferative assay		48 hrs	Antiproliferative activity against human RKO cells after 48 hrs by XTT assay, IC50 = 0.11 μM.	20537765
RKOp27	Cytotoxicity assay		48 hrs	Cytotoxicity against non induced human RKOp27 cells after 48 hrs by XTT assay, IC50 = 0.11 μM.	21705223
SW480	Antiproliferative assay		48 hrs	Antiproliferative activity against human SW480 cells after 48 hrs by alamar blue assay, IC50 = 0.12 μM.	21563750
A549	Growth inhibition assay		72 hrs	Growth inhibition of human A549 cells after 72 hrs by MTT assay, IC50 = 0.12 μM.	25262051
KB/HeLa	Antiproliferative assay			Antiproliferative activity against human KB/HeLa cells by XTT assay, IC50 = 0.14 μM.	19220018
KB/HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against KB/HeLa cells by XTT assay after 48 hrs, IC50 = 0.14 μM.	17181164
KB/HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM.	17973361
KB/HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM.	20537765
KB/HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM.	21705223
HT1080	Growth inhibition assay		72 hrs	Growth inhibition of human HT1080 cells after 72 hrs by MTT assay, IC50 = 0.14 μM.	25262051
NCI-H460	Antiproliferative assay		48 hrs	Antiproliferative activity against NCI-H460 cells by XTT assay after 48 hrs, IC50 = 0.15 μM.	17181164
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM.	17973361
NCI-H460	Antiproliferative assay			Antiproliferative activity against human NCI-H460 cells by XTT assay, IC50 = 0.15 μM.	19220018
NCI-H460	Antiproliferative assay		48 hrs	Antiproliferative activity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM.	20537765
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM.	21705223
SKOV3	Antiproliferative assay		48 hrs	Antiproliferative activity against SKOV3 cells by XTT assay after 48 hrs, IC50 = 0.17 μM.	17181164
P388 ADR	Antiproliferative assay		48 hrs	Antiproliferative activity against P388 ADR cells by XTT assay after 48 hrs, IC50 = 0.17 μM.	17181164
SKOV3	Cytotoxicity assay		48 hrs	Cytotoxicity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM.	17973361
SKOV3	Antiproliferative assay			Antiproliferative activity against human SKOV3 cells by XTT assay, IC50 = 0.17 μM.	19220018
SKOV3	Antiproliferative assay		48 hrs	Antiproliferative activity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM.	20537765
P388	Antiproliferative assay		48 hrs	Antiproliferative activity against adriamycin-resistant mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.17 μM.	20537765
SKOV3	Cytotoxicity assay		48 hrs	Cytotoxicity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM.	21705223
HCT116	Cytotoxicity assay			Cytotoxicity against human HCT116 cells by SRB assay, IC50 = 0.173 μM.	21954851
HeLa	Cytotoxicity assay		24 to 48 hrs	Cytotoxicity against human HeLa cells after 24 to 48 hrs by trypan blue assay, IC50 = 0.178 μM.	23445405
L1210	Function assay	0.3 uM	24 hrs	Fraction of cell population of cultured lymphoid leukemia L1210 cells in mitosis at a dose of 0.3 uM after 24 hr, Mitotic index = 0.19 μM.	7131483
DLD1	Antiproliferative assay		48 hrs	Antiproliferative activity against human DLD1 cells after 48 hrs by alamar blue assay, IC50 = 0.19 μM.	21563750
SF268	Antiproliferative assay		48 hrs	Antiproliferative activity against SF268 cells by XTT assay after 48 hrs, IC50 = 0.3 μM.	17181164
SF268	Cytotoxicity assay		48 hrs	Cytotoxicity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM.	17973361
SF268	Antiproliferative assay			Antiproliferative activity against human SF268 cells by XTT assay, IC50 = 0.3 μM.	19220018
L12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat L12 cells after 48 hrs by XTT assay, IC50 = 0.3 μM.	17973361
SF268	Antiproliferative assay		48 hrs	Antiproliferative activity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM.	20537765
SF268	Cytotoxicity assay		48 hrs	Cytotoxicity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM.	21705223
LT12	Antiproliferative assay		48 hrs	Antiproliferative activity against rat LT12 cells ectopic expression of human MDR1 after 48 hrs by XTT assay, IC50 = 0.4 μM.	19220018
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.5 μM.	20529689
SK-N-SH	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-N-SH cells after 48 hrs by SRB assay, GI50 = 0.65 μM.	20529689
SK-N-SH	Growth inhibition assay			Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay, GI50 = 0.66 μM.	21106458
HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50 = 0.71 μM.	23748152
HeLa	Growth inhibition assay			Growth inhibition of human HeLa cells by Sulforhodamine B assay, GI50 = 0.75 μM.	21106458
SK-N-SH	Growth inhibition assay			Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay, LC50 = 0.75 μM.	21106458
K562	Function assay			Inhibition of tubulin polymerization in human K562 cells, IC50 = 0.76 μM.	20546980
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.81 μM.	25131956
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.81 μM.	27344493
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 0.89 μM.	25599948
HeLa	Growth inhibition assay			Growth inhibition of human HeLa cells by Sulforhodamine B assay, LC50 = 0.9 μM.	21106458
A549	Antiproliferative assay		48 hrs	Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, IC50 = 0.9 μM.	28988624
MDA-MB-453	Cytotoxicity assay		48 hrs	Cytotoxicity in human MDA-MB-453 cells incubated for 48 hrs by MTT assay, IC50 = 0.91 μM.	28923381
MCF7	Antiproliferative assay		48 hrs	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.94 μM.	25131956
MCF7	Antiproliferative assay		48 hrs	Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay, GI50 = 0.94 μM.	27344493
MIAPaCa2	Antiproliferative assay		48 hrs	Antiproliferative activity against human MIAPaCa2 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.95 μM.	25131956
MIAPaCa2	Antiproliferative assay		48 hrs	Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by SRB assay, GI50 = 0.95 μM.	27344493
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells after 48 hrs by SRB assay, LC50 = 1 μM.	20529689
HCT116	Cytotoxicity assay		48 hrs	Cytotoxicity in human HCT116 cells incubated for 48 hrs by MTT assay, IC50 = 1.06 μM.	28923381
HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 1.1 μM.	25599948
DU145	Cytotoxicity assay		48 hrs	Cytotoxicity in human DU145 cells incubated for 48 hrs by MTT assay, IC50 = 1.14 μM.	28923381
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, IC50 = 1.2 μM.	28988624
MCF7	Antiproliferative assay		48 hrs	Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay, IC50 = 1.25 μM.	28988624
HCT116	Anticancer assay		48 hrs	Anticancer activity against human HCT116 cells after 48 hrs by MTT assay, IC50 = 1.262 μM.	28789894
IMR32	Antiproliferative assay		48 hrs	Antiproliferative activity against human IMR32 cells after 48 hrs by SRB assay, IC50 = 1.3 μM.	28988624
A549	Anticancer assay		48 hrs	Anticancer activity against human A549 cells after 48 hrs by MTT assay, IC50 = 1.513 μM.	28789894
MCF7	Anticancer assay		48 hrs	Anticancer activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 1.6 μM.	26231080
DU145	Anticancer assay		48 hrs	Anticancer activity against human DU145 cells after 48 hrs by MTT assay, IC50 = 1.698 μM.	28789894
MCF7	Cytotoxicity assay		48 hrs	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 1.7 μM.	25599948
PC3	Cytotoxicity assay		48 hrs	Cytotoxicity in human PC3 cells incubated for 48 hrs by MTT assay, IC50 = 1.84 μM.	28923381
MDA-MB-231	Anticancer assay		48 hrs	Anticancer activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 1.862 μM.	28789894
A549	Anticancer assay		48 hrs	Anticancer activity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 1.87 μM.	26231080
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay, IC50 = 1.87 μM.	28668476
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells after 48 hrs by MTT assay, IC50 = 2.31 μM.	23748152
SK-N-SH	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-N-SH cells after 48 hrs by SRB assay, LC50 = 2.8 μM.	20529689
DU145	Cytotoxicity assay		48 hrs	Cytotoxicity against human DU145 cells after 48 hrs by MTT assay, IC50 = 2.8 μM.	23748152
HeLa	Anticancer assay		48 hrs	Anticancer activity against human HeLa cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 2.83 μM.	26231080
HeLa	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 48 hrs by MTT assay, IC50 = 2.83 μM.	28668476
DU145	Cytotoxicity assay		48 hrs	Cytotoxicity against human DU145 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 3.2 μM.	25599948
HaCaT	Cytotoxicity assay		48 hrs	Cytotoxic activity against human HaCaT cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 8.9 μM.	26231080
NIH3T3	Apoptosis assay	0.5 ug/ml	24 hrs	Induction of apoptosis in mouse NIH3T3 cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry	18247573
PC3M	Apoptosis assay	0.5 ug/ml	24 hrs	Induction of apoptosis in human PC3M cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry	18247573
NRK	Function assay	30 uM	60 mins	Golgi-disturbing activity in golgi apparatus of rat NRK cells assessed as microtubule depolymerization-associated golgi fragmentation at 30 uM after 60 mins by Hoechst 3342 staining-based immunofluorescence microscopy	20189813
Vero	Function assay	5 ug/ml	30 mins	Effect on disruption of microtubules in african green monkey Vero cells at 5 ug/ml after 30 mins by immunofluorescence method	19182783
MCF7	Apoptosis assay	2.5 uM	48 hrs	Induction of apoptosis in human MCF7 cells assessed as nuclear fragmentation at 2.5 uM after 48 hrs by DAPI staining	21194809
MCF7	Function assay	2.5 uM	48 hrs	Inhibition of tubulin polymerization in human MCF7 cells assessed as disrupted microtubule organization at 2.5 uM after 48 hrs by microscopic analysis	21194809
A549	Cell cycle assay		24 hrs	Cell cycle arrest in human A549 cells assessed as accumulation of cells in G2 phase after 24 hrs measured using propidium iodide staining by FACS analysis	21863853
A549	Apoptosis assay	10 to 100 nM	48 hrs	Induction of apoptosis in human A549 cells assessed as early apoptotic cells at 10 to 100 nM after 48 hrs by annexin-V FITC/7-ADD staining-based flow cytometric analysis	21916509
A549	Apoptosis assay	10 to 100 nM	48 hrs	Induction of apoptosis in human A549 cells assessed as necrotic cells at 10 to 100 nM after 48 hrs by annexin-V FITC/7-ADD staining-based flow cytometric analysis	21916509
A549	Cell cycle assay	1 uM	48 hrs	Cell cycle arrest in human A549 cells assessed as accumulation at G2/M phase at 1 uM after 48 hrs by propidium iodide staining-based flow cytometric analysis relative to control	22361684
SKBR3	Function assay	50 to 100 nM	24 hrs	Induction of Hsp27 phosphorylation in human SKBR3 cells at 50 to 100 nM after 24 hrs by SDS-PAGE analysis	22435708
SKBR3	Function assay	0.2 to 1 uM	24 hrs	Induction of Hsp27 phosphorylation in human SKBR3 cells at 0.2 to 1 uM after 24 hrs by SDS-PAGE analysis	22435708
MCF7	Function assay	4 uM	24 hrs	Inhibition of microtubule organization in human MCF7 cells at 4 uM after 24 hrs by immunofluorescence analysis	22982122
Jurkat T	Function assay		36 hrs	Induction of microtubule damage in human Jurkat T cells overexpressing Bcl2 after 36 hrs by immunostaining method	22197393
Jurkat T	Function assay		36 hrs	Induction of microtubule damage in human Jurkat T cells overexpressing Neo after 36 hrs by immunostaining method	22197393
Jurkat T	Apoptosis assay		36 hrs	Induction of apoptosis in human Jurkat T cells overexpressing Bcl2 assessed as cellular protrusions after 36 hrs by light microscopy	22197393
Jurkat T	Apoptosis assay		36 hrs	Induction of apoptosis in human Jurkat T cells overexpressing Neo after 36 hrs by flow cytometry	22197393
Jurkat T	Apoptosis assay		36 hrs	Induction of apoptosis in human Jurkat T cells overexpressing Neo assessed as cellular protrusions after 36 hrs by light microscopy	22197393
HeLa	Function assay	1 uM	24 hrs	Inhibition of tubulin polymerization in human HeLa cells assessed as tubulin soluble fraction at 1 uM after 24 hrs by immunohistochemistry analysis	23313639
HeLa	Function assay	1 uM	24 hrs	Mitotic arrest in human HeLa cells assessed as increase in accumulation at G2/M phase at 1 uM for 24 hrs by PI staining/FACS analysis	24530033
HeLa	Cell cycle assay	2 uM	24 hrs	Cell cycle arrest at G2/M phase in human HeLa cells assessed as increase in cyclin B1 expression at 2 uM after 24 hrs by immunoblot analysis relative to control	25499929
HeLa	Cell cycle assay	2 uM	24 hrs	Cell cycle arrest at G2/M phase in human HeLa cells assessed as soluble form of tubulin at 2 uM after 24 hrs by immunoblot analysis relative to control	25499929
DU145	Function assay	50 uM	48 hrs	Disruption of microtubule network in human DU145 cells at 50 uM after 48 hrs by immunohistochemistry analysis	25615796
HeLa	Function assay	25 uM	24 hrs	Effect on PCM1 protein in human HeLa cells assessed as accumulation of pericentriolar material at 25 uM after 24 hrs by immunofluorescence microscopy	25050880
SGC7901	Function assay		48 hrs	Induction of distortions in tubulin assembly in human SGC7901 cells assessed as abnormal mitotic spindle formation at 2 fold IC50 level incubated for 48 hrs by immunofluorescence staining based fluorescence microscopy	25262051
HT1080	Function assay		48 hrs	Induction of distortions in tubulin assembly in human HT1080 cells assessed as abnormal mitotic spindle formation at 2 fold IC50 level incubated for 48 hrs by immunofluorescence staining based fluorescence microscopy	25262051
MCF7	Function assay	1 uM	4 hrs	Induction of tubulin depolymerization in human MCF7 cells at 1 uM after 4 hrs by sedimentation assay and Western blotting method	25369367
DU145	Function assay	1.5 uM	48 hrs	Reduction in mitochondrial membrane potential in human DU145 cells at 1.5 uM incubated for 48 hrs by JC1 dye staining based flow cytometry	26330077
SKBR3	Function assay	2.5 uM	24 hrs	Induction of microtubule destabilization in human SKBR3 cells assessed as soluble fraction of protein at 2.5 uM after 24 hrs by Western blot analysis	26599530
HeLa	Function assay	100 nM	4 hrs	Inhibition of tubulin polymerization in human HeLa cells assessed as microtubule disruption in mitotic phase at 100 nM after 4 hrs by indirect immunofluorescence analysis	26641132
HeLa	Function assay	100 nM	4 hrs	Inhibition of tubulin polymerization in human HeLa cells assessed as microtubule disruption in interphase at 100 nM after 4 hrs by indirect immunofluorescence analysis	26641132
TC32	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells	29435139
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
SJ-GBM2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
NB-EBc1	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
Saos-2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
BT-12	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
OHS-50	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB1643 cells	29435139
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells)	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells	29435139
BT-12	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells	29435139
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for DAOY cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells	29435139
TC32	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells	29435139
MG 63 (6-TG R)	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells	29435139
U-2 OS	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for U-2 OS cells	29435139
RD	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for RD cells	29435139
Saos-2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Saos-2 cells	29435139
OHS-50	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells	29435139
HeLa	Function assay		24 hrs	Inhibition of tubulin polymerization in human HeLa cells assessed as increase in soluble tubulin fraction after 24 hrs by Western blot analysis	28818768
HeLa	Function assay	0.1 uM	24 hrs	Inhibition of tubulin polymerization in human HeLa cells assessed as shift of tubulin protein to soluble fraction at 0.1 uM after 24 hrs by Western blot analysis	29501940
PC3MLN4	Function assay	1 uM	24 hrs	Upregulation of Cyclin-B1 protein expression in human PC3MLN4 cells at 1 uM after 24 hrs by Western blot method	29288939
PC3MLN4	Function assay	1 uM	48 hrs	Disruption of microtubule dynamics in human PC3MLN4 cells at 1 uM after 48 hrs by immunofluorescence microscopic method	29288939
MCF7	Function assay	1 uM	4 hrs	Induction of tubulin depolymerization in human MCF7 cells at 1 uM after 4 hrs by Western blot method	27689728
Jurkat	Function assay	1 uM	4 hrs	Induction of tubulin depolymerization in human Jurkat cells at 1 uM after 4 hrs by Western blot method	27689728
Cliquez pour voir plus de données expérimentales sur les lignées cellulaires

Informations chimiques, stockage et stabilité

Poids moléculaire	301.32	Formule	C₁₄H₁₁N₃O₃S	Stockage (À partir de la date de réception)	3 ans-20°Cpoudre
N° CAS	31430-18-9	Télécharger le SDF		Stockage des solutions mères	1 an-80°Cen solvant 1 mois-20°Cen solvant
Synonymes	R17934, Oncodazole, NSC238159			Smiles	COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3

Solubilité

In vitro
Lot:

DMSO : 7.5 mg/mL (24.89 mM)
(Le DMSO contaminé par lhumidité peut réduire la solubilité. Utiliser du DMSO frais et anhydre.)

Water : Insoluble

Ethanol : Insoluble

Calculateur de molarité

Masse	Concentration	Volume	Poids moléculaire
=	×	×

Calculateur de dilution Calculateur de poids moléculaire

In vivo Lot:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validé par les laboratoires Selleck. Si vous avez besoin dajustements à cette formulation, contactez notre équipe de vente pour des tests personnalisés.	0.380mg/ml (1.26mM)	Taking the 1 mL working solution as an example, add 50 μL 7.5 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to make it clear. Volume up to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Calculateur de formulation in vivo (Solution claire)

Étape 1 : Entrez les informations ci-dessous (Recommandé : Un animal supplémentaire pour tenir compte des pertes pendant lexpérience)

Dosage : mg/kg Poids moyen des animaux : g Volume de dosage par animal :μL Nombre danimaux :

Étape 2 : Entrez la formulation in vivo (Ceci nest que le calculateur, pas la formulation. Veuillez nous contacter dabord sil ny a pas de formulation in vivo dans la section Solubilité.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Résultats du calcul :

Concentration de travail : mg/ml;

Méthode de préparation du liquide maître DMSO : mg médicament prédissous dans μL DMSO ( Concentration du liquide maître mg/mL, Veuillez nous contacter dabord si la concentration dépasse la solubilité du DMSO du lot de médicament. )

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, ajouter ensuiteμL PEG300, mélanger et clarifier, ajouter ensuiteμL Tween 80, mélanger et clarifier, ajouter ensuite μL ddH₂O, mélanger et clarifier.

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, ajouter ensuite μL Huile de maïs, mélanger et clarifier.

Remarque : 1. Assurez-vous que le liquide est clair avant dajouter le solvant suivant.
2. Assurez-vous dajouter le(s) solvant(s) dans lordre. Vous devez vous assurer que la solution obtenue lors de lajout précédent est une solution claire avant de procéder à lajout du solvant suivant. Des méthodes physiques telles que le vortex, les ultrasons ou le bain-marie peuvent être utilisées pour faciliter la dissolution.

Mécanisme daction

Targets/IC₅₀/Ki	Microtubules (Cell-free assay) Abl (Cell-free assay) 0.21 μM Abl (E255K) (Cell-free assay) 0.53 μM Abl (T315I) (Cell-free assay) 0.64 μM
In vitro	Nocodazole est un ligand de haute affinité pour les kinases liées au cancer, y compris Abl phosphorylée, c-Kit, BRAF et MEK avec un K_d de 0,091 μM, 1,6 μM, 1,8 μM et 1,6 μM, respectivement. De plus, le K_d de ce composé pour Abl(E255K) phosphorylée, Abl(T315I) phosphorylée, BRAF(V600E) et PI3Kγ est de 0,12 μM, 0,17 μM, 1,1 μM et 1,5 μM, respectivement. Il induit l'apoptosis dans les cellules de leucémie lymphoïde chronique. Cette substance chimique inhibe le transport du glucose stimulé par l'insuline. Elle diminue l'apoptosis dans certaines cellules de carcinome du côlon humain. Elle altère la morphologie et la directionnalité des cellules de l'éminence ganglionnaire médiale migrantes. À des concentrations élevées, ce composé dépolymérise rapidement les microtubules dans les cellules, tandis que de faibles concentrations de celui-ci inhibent l'instabilité dynamique des microtubules. Les cellules mitotiques incubées avec différentes concentrations sont inhibées de progresser en phase G₁ 6 heures après la libération du bloc de Nocodazole, avec une concentration inhibitrice médiane de 4 nM. Les cellules prétraitées au Nocodazole exposées en l'absence de cette substance chimique ne forment que des microtubules flottants libres, tandis que les cellules prétraitées exposées en sa présence organisent des microtubules assemblés au centrosome. Il perturbe les microtubules en se liant à la β-tubuline. Ce composé empêche la formation de l'une des deux liaisons disulfures inter-chaînes. Il altère le transport des vésicules. Il supprime la mort cellulaire et la dysfonction lysosomale induites par le METH. La mort cellulaire induite par le METH est significativement diminuée par le prétraitement avec ce composé par rapport au METH seul. Il double l'efficacité HDR jusqu'à 30% dans les iPSCs. Il améliore l'efficacité HDR médiée par CRISPR et a un effet additif sur l'amélioration de l'édition précise du génome.
In vivo	Le volume et le poids tumoraux des souris traitées avec Nocodazole sont significativement réduits par rapport à ceux traités avec ce composé seul. Le traitement combiné avec cette substance chimique améliore fortement l'apoptosis des xénogreffes tumorales COLO 205 traitées avec celle-ci seule.
Références	[1] https://pubmed.ncbi.nlm.nih.gov/22002881/ [2] http://onlinelibrary.wiley.com/doi/10.1002/ddr.10023/abstract [3] https://pubmed.ncbi.nlm.nih.gov/7913875/ [4] https://pubmed.ncbi.nlm.nih.gov/22613348/ [5] https://pubmed.ncbi.nlm.nih.gov/12203371/ [6] https://pubmed.ncbi.nlm.nih.gov/28219395/ [7] https://pubmed.ncbi.nlm.nih.gov/34388376/

Applications

Méthodes	Biomarqueurs	PMID
Western blot	Cdc2 / cyclin B1 / MAD2 / Cdc20	21918689
Immunofluorescence	Tubulin / LC8 Brd4 / α-tubulin	23038268
Growth inhibition assay	Cell viability	21918689

Support technique

Instructions de manipulation

Tel: +1-832-582-8158 Ext:3

Si vous avez dautres questions, veuillez laisser un message.

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