DMOG (Dimethyloxallyl Glycine)

N.º de catálogoS7483 Lote:S748304

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Datos técnicos

Fórmula

C6H9NO5
Peso molecular 175.14 Número CAS 89464-63-1
Solubilidad (25°C)* In vitro DMSO 35 mg/mL (199.84 mM)
Water 35 mg/mL (199.84 mM)
Ethanol 35 mg/mL (199.84 mM)
In vivo (Agregue los solventes al producto individualmente y en orden.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml significa ligeramente soluble o insoluble.
* Tenga en cuenta que Selleck prueba la solubilidad de todos los compuestos internamente, y la solubilidad real puede diferir ligeramente de los valores publicados. Esto es normal y se debe a ligeras variaciones entre lotes.
* Envío a temperatura ambiente (Las pruebas de estabilidad demuestran que este producto se puede enviar sin medidas de refrigeración.)

Preparación de soluciones madre

Actividad biológica

Descripción DMOG (Dimethyloxalylglycine) es un antagonista del cofactor α-cetoglutarato e inhibidor de la HIF prolylhydroxylase, lo que lleva a la estabilización y acumulación de la proteína HIF-1α en el núcleo. DMOG mejora la autophagy.Las soluciones son inestables y deben prepararse frescas.
Objetivos
HIF prolyl hydroxylase
In vitro

DMOG shows only weakly active in the microsomal system, but efficiently suppresses hydroxyproline synthesis in intact cells. This compound reduces FGF-2-induced proliferation and cyclin A expression by inhibiting prolyl hydroxylase activity in HPASMC.

In vivo

DMOG inhibits endogenous HIF inactivation, and induces angiogenesis in ischaemic skeletal muscles of mice. Up-regulation of hypoxia-inducible factor-1α by this compound enhances the cardioprotective effects of ischemic postconditioning in hyperlipidemic rats.

Densidad 1.246 g/mL

Protocolo (de referencia)

Ensayo celular:

[3]
  • Líneas celulares

    VSMC cells

  • Concentraciones

    ~100 μM

  • Tiempo de incubación

    72-96 hours

  • Método

    To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells.
Estudio en animales:

[2]
  • Modelos animales

    Mice (C57Bl6)

  • Dosificaciones

    8 mg in 0.5 ml saline

  • Administración

    i.p.

Referencias

  • https://pubmed.ncbi.nlm.nih.gov/8002959/
  • https://pubmed.ncbi.nlm.nih.gov/15319416/
  • https://pubmed.ncbi.nlm.nih.gov/19304911/
  • https://pubmed.ncbi.nlm.nih.gov/24389644/

Validación de productos por parte del cliente

Western blot was performed to analyze p65 signaling and the expression of Bcl2 and cleaved Caspase 3. GAPDH served as the loading control

Datos de [ , , Hepatology, 2018, 68(6):2359-2375 ]

Western blot analysis of the indicated proteins after the treatment with DMOG (K).

Datos de [ , , Theranostics, 2017, 7(13): 3293-3305 ]

Western blot analysis of the indicated proteins after the treatment with DMOG.

Datos de [ , , Theranostics, 2017, 7(13):3293-3305 ]

OCI‐LY7 cells were cultured with DMOG (1·5 mM) treatment for 96 h, bortezomib (5 nM), with combined treatment by bortezomib (5 nM) for another 5 h, after being pretreated with DMOG (1·5 mM) for 91 h. Apoptosis of AIDWT (upper) and AIDKO (lower) was assessed by detecting signals of the apoptosis maker annexin V and 7‐aminoactinomycin D (7‐AAD) (left); and statistical diagram of percentage for apoptosis cells (right). Bor = bortezomib.

Datos de [ , , Clin Exp Immunol, 2018, doi:10.1111/cei.13227 ]

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