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ISX-9 (Isoxazole 9) Inductor de la diferenciación de células madre neurales

Name: ISX-9 (Isoxazole 9)
Brand: Selleck Chemicals
SKU: S7914
Rating: 5 (8 reviews)

Cat. No.: S7914

ISX-9 (Isoxazole 9) es un promotor sintético de la neurogénesis adulta al desencadenar la diferenciación neuronal de células madre/precursoras neurales adultas (NSPC). ISX-9 (Isoxazole 9) activa múltiples vías, incluyendo la señalización de la transición epitelio-mesenquimal (EMT) inducida por TGF-β, la señalización Wnt canónica y no canónica en diferentes etapas de la diferenciación cardíaca.

ISX-9 (Isoxazole 9) Activador Chemical Structure

Estructura química

Peso molecular: 234.27

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Control de calidad (Quality Control)

Lote: Pureza: 99.87%

99.87

Dianas relacionadas	JAK TGF-beta/Smad Wnt/beta-catenin ERK GSK-3 ROCK Hedgehog/Smoothened PKA Secretase STAT
Otros Inhibidores	YAP-TEAD Inhibitor 1 (Peptide 17) L-Buthionine-(S,R)-sulfoximine (L-BSO) PF-3644022 WAY-641966 WAY-320461

Cultivo celular, tratamiento y concentración de trabajo
(Cell Culture, Treatment & Working Concentration)

Líneas celulares	Tipo de ensayo	Concentración	Tiempo de incubación	Descripción de la actividad	PMID
HCN	Function assay			Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence of NMDA receptor antagonist MK801	18552832
HCN	Function assay	5 uM		Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of multiple of inhibitors	18552832
HCN	Function assay	50 uM	3 hrs	Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801	18552832
HCN	Function assay	25 uM	4 days	Inhibition of gliogenesis differentiation in rat HCN cells at 25 uM after 4 days	18552832
HCN	Function assay	5 uM		Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis	18552832
HCN	Function assay	5 uM		Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of NMDA receptor antagonist MK801	18552832
HCN	Function assay	50 uM	3 hrs	Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of multiple inhibitors	18552832
HCN	Function assay			Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells	18552832
HCN	Function assay			Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence multiple inhibitors	18552832
HCN	Function assay	50 uM	24 hrs	Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801	18552832
HCN	Function assay	50 uM	24 hrs	Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine	18552832
HCN	Function assay	50 uM	24 hrs	Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of multiple inhibitors	18552832
HCN	Function assay	8 to 64 uM	9 hrs	Induction of neurogenesis in undifferentiated rat HCN cells assessed as MAP2AB protein level at 8 to 64 uM after 9 hrs by protein blotting analysis	18552832
HCN	Function assay			Induction of HDAC5 translocation in cytoplasm of rat HCN cells assessed as phosphorylated HDAC5 accumulation	18552832
HCN	Function assay			Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 2.5 uM potassium channel inhibitor KN92	18552832
HCN	Function assay	8 to 64 uM	9 hrs	Induction of neurogenesis in undifferentiated rat HCN cells assessed as GlR2/3 protein level at 8 to 64 uM after 9 hrs by protein blotting analysis	18552832
HCN	Function assay		6 hrs	Induction of HDAC5 phosphorylation in rat HCN cells after 6 hrs	18552832
HCN	Function assay		24 hrs	Induction of HDAC5 phosphorylation in rat HCN cells after 24 hrs	18552832
HCN	Function assay			Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as phosphorylated HDAC5 accumulation	18552832
HCN	Function assay	5 uM		Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 S258A S498A mutant fusion protein accumulation at 5 uM by fluorescence assay	18552832
HCN	Function assay	50 uM	3 hrs	Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine	18552832
HCN	Function assay	20 uM		Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 fusion protein accumulation at 20 uM by fluorescence assay	18552832
HCN	Function assay			Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 200 nM PKC inhibitor Go6976	18552832
HCN	Function assay		24 hrs	Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of multiple inhibitors	18552832
HCN	Function assay		24 hrs	Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist MK801	18552832
HCN	Function assay		24 hrs	Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist nifedipine	18552832
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Información química, almacenamiento y estabilidad (Chemical Information, Storage & Stability)

Peso molecular	234.27	Fórmula	C₁₁H₁₀N₂O₂S	Almacenamiento (Desde la fecha de recepción)	3 años-20°Cpolvo
Nº CAS	832115-62-5	Descargar SDF		Almacenamiento de soluciones madre	1 año-80°Cen disolvente 1 mes-20°Cen disolvente
Sinónimos	N/A			Smiles	C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3

Solubilidad (Solubility)

In vitro
Lote:

DMSO : 47 mg/mL (200.62 mM)
(El DMSO contaminado con humedad puede reducir la solubilidad. Usar DMSO fresco y anhidro.)

Ethanol : 12 mg/mL

Water : Insoluble

Calculadora de Molaridad

Masa	Concentración	Volumen	Peso molecular
=	×	×

Calculadora de Dilución Calculadora de Peso Molecular

In vivo Lote:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 55%PEG300 2 40%ddH2O Validado por los laboratorios Selleck. Si necesita ajustes en esta formulación, contacte con nuestro equipo de ventas para pruebas personalizadas.	10.000mg/ml (42.69mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 550 μL of PEG 300, mix evenly to clarify it; then continue to add 400 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Calculadora de formulación in vivo (Solución clara)

Paso 1: Introduzca la información a continuación (Recomendado: Un animal adicional para tener en cuenta la pérdida durante el experimento)

Dosis: mg/kg Peso promedio de los animales: g Volumen de dosificación por animal:μL Número de animales:

Paso 2: Introduzca la formulación in vivo (Esto es solo la calculadora, no la formulación. Por favor, contáctenos primero si no hay una formulación in vivo en la sección de Solubilidad.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Resultados del cálculo:

Concentración de trabajo: mg/ml;

Método para preparar el líquido maestro de DMSO: mg fármaco predissuelto en μL DMSO ( Concentración del líquido maestro mg/mL, Por favor, contáctenos primero si la concentración excede la solubilidad del DMSO del lote del fármaco. )

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadirμL PEG300, mezclar y clarificar, luego añadirμL Tween 80, mezclar y clarificar, luego añadir μL ddH₂O, mezclar y clarificar.

Método para preparar la formulación in vivo: Tomar μL DMSO líquido maestro, luego añadir μL Aceite de maíz, mezclar y clarificar.

Nota: 1. Por favor, asegúrese de que el líquido esté claro antes de añadir el siguiente disolvente.
2. Asegúrese de añadir el (los) disolvente(s) en orden. Debe asegurarse de que la solución obtenida, en la adición anterior, sea una solución clara antes de proceder a añadir el siguiente disolvente. Se pueden utilizar métodos físicos como el vórtice, el ultrasonido o el baño de agua caliente para ayudar a la disolución.

Mecanismo de acción (Mechanism of Action)

In vitro	Isoxazole 9 (ISX-9) increases cell number and promotes cell differentiation in NSPCs, whereas it induces cell damage in OPCs. In outgrowth EPCs, this compound decreases tube formation without effect on early EPCs.
In vivo	In mice, isoxazole 9 (ISX-9) (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. This compound also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, it improves spatial memory.
Referencias	[1] https://pubmed.ncbi.nlm.nih.gov/26407349/ [2] https://pubmed.ncbi.nlm.nih.gov/22542682/

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